Monoclonal Antibodies against Platelet Membrane Glycoproteins. Characterization and Effect on Platelet Function
نویسندگان
چکیده
منابع مشابه
Monoclonal Antibodies Against Porcine Platelet Membrane Glycoproteins lb and
We prepared murine monoclonal antibodies to porcine platelet membrane glycoprotein (GP) lb and GP llb/Illa for further study of the porcine hemostatic mechanism. One monoclonal antibody, designated PP3-4C. blocked Ristocetin-induced platelet agglutination and caused 80% inhibition of Ristocetin-induced ‘25l-von Willebrand factor (vWF) binding to porcine platelets at a concentration of 1 2 gsg l...
متن کاملEffect of monoclonal antibodies against von Willebrand factor and platelet glycoproteins IIb/IIIa on the platelet retention test.
The platelet retention test provides a measure of the number of platelets retained in a column of glass beads and is one of the few in vitro platelet function tests that is abnormal in von Willebrand's disease (vWd). In a two-stage test, 1 mL of blood (designated A) was passed through the column, followed by 5 mL of isotonic saline and then 5 mL of blood (B) in which platelet retention was meas...
متن کاملDetection and characterization of monoclonal antibodies to platelet membrane proteins
We have devised a solid-phase radioimmunoassay for the detection and characterization of monoclonal antibodies directed against platelet surface antigens. Platelet membrane proteins, solubilized with 0.1% Triton X-100, were covalently coupled to cyanogen bromide (CNBr)-activated filter paper disks that were than used as the support in antibody binding assays. SDS PAGE of solubilized membrane pr...
متن کاملEffect of Monoclonal Antibodies Against von Willebrand Factor and Platelet
The platelet retention test provides a measure of the number of platelets retained in a column of glass beads and is one of the few in vitro platelet function tests that is abnormal in von Willebrand’s disease (vWd). In a two-stage test, 1 mL of blood (designated A) was passed through the column, followed by 5 mL of isotonic saline and then 5 mL of blood (B) in which platelet retention was meas...
متن کامل974 Platelet Membrane Glycoproteins
Glycoproteins I Ib / I I Ia form a Ca2+-dependent complex that functions as the fibrinogen receptor on human platelets (1). Several reports have indicated that this fibrinogen receptor is also expressed on the surface of human monocytes (2-4). Of three monoclonal antibodies directed against platelet I Ib/ I I Ia , two were reported to give a positive reaction with monocytes as well (4). Other i...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1983
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1983.tb07281.x